UA Biophysics:Protocols:Ampicillin

Ampicillin sodium salt 50 mg/ml

Ampicillin is a ß-lactam antibiotic that inhibits bacterial cell-wall synthesis by inactivating transpeptidases on the inner surface of the bacterial cell membrane. Ampicillin in solution is a function of pH, temperature and the identity of the buffer. It’s activity is quickly lost when stored above pH 7.

Materials

• Ampicillin sodium salt sc-202951
• Sterilized ddH₂O
• Syringe filter
• Syringe 50 ml

Protocol

1. According to desired amount of antibiotics, calculate the amount in grams that has to be weighed. For example, for 10mL of 25mg/mL of Ampicillin: 25mg/mL*10mL=250mg
2. Ampicillin is soluble in ddH₂O.
3. Filter the solution using a 0.2 µm syringe filter.
4. Divide in aliquots of 500μL
5. Store at -20 °C. up to 4-6 months

"It is stable at 37°C for three days. Additional studies have shown that the stability of Ampicillin in solution is a function of pH, temperature and the identity of the buffer. It′s activity is quickly lost when stored above pH 7. " Sigma

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