UA Biophysics:Protocols:Erythromycin

Erytromicin 1 mg/ml

Erythromycin acts by inhibiting elongation at the transpeptidation step, specifically aminoacyl translocation from the A-site to P-site by binding to the 50s subunit of the bacterial 70s rRNA complex.

Materials

• Erythromycin E5389-1G
• Ethanol
• Syringe filter
• Syringe 50 ml

Protocol

1. According to desired amount of antibiotics, calculate the amount in grams that has to be weighed. For example, for 10mL of 25mg/mL of Ampicillin: 25mg/mL*10mL=250mg
2. Weigh the Erythromycin
3. Erythromycin has to be diluted in EtOH (molecular grade)
4. Filter the solution using a 0.2 µm syringe filter.
5. Divide in aliquots of 500μL
6. Store at -20 °C

Erythromycin is light sensitive. Keep tightly closed in light-resistant containers. This product is stable in solution at 37°C for 3 days. Stock solutions should be stored at 2-8°C.

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